Welcome to Webcutter 2.0! This new version of Webcutter is a
complete rewrite. Along with cleaner and more maintainable code, I am
pleased to introduce the following new features:
Rainbow
cutters Highlight your favorite enzymes in color or boldface for easy
at-a-glance identification
Silent cutters Find sites which may
be introduced by silent mutagenesis of your coding sequence
Sequence uploads Input sequences directly into Webcutter from a
file on your hard drive without needing to cut-and-paste
Degenerate
sequences Analyze restriction maps of sequences containing ambiguous
nucleotides like N, Y, and R.
Circular sequences Choose whether
to treat your sequence as linear or circular
Enzyme info Click
into the wealth of references and ordering information at New England
BioLabs' REBASE, directly from your restriction map results
Plus all
of the features Webcutter has always had, from automatic sequence
search-and-entry from NCBI's GenBank to its easy customizable interface
and clean simple results format. For a mini-manual on how Webcutter 2.0
works, how to get the most from it, and some of its known limitations,
please click here.
New!
From the author of
Webcutter Webminer, an intuitive but powerful tool
for searching yeast genomic expression databases. Please give it a try!
There are three ways to input your sequence:
You can copy-and-paste it or type it into the box below
You can upload a sequence file from your computer by clicking the "Browse..." button below. If you are not using Netscape, you may not see the browse button and cannot use this feature (sorry).
Or, you can search NCBI's GenBank for your sequence by entering search terms below. Webcutter will submit your search to GenBank and automatically retrieve and enter your sequence for you.
For more advanced searches, please visit one of these sites